Isolation and taxonomic characterization of medicinal mushroom Ganoderma spp
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چکیده
The present study deals with isolation, taxonomic characterization and biological activity of medicinal mushroom Ganoderma spp. The results of the present study Ganoderma sp. DKR1 and Ganoderma sp. DKR2 were isolated from two hard woods of Casurina equiestifolia and Morinda tinctoria respectively in Cuddalore district, Tamil Nadu, India. The Ganoderma isolate were characterized for macro and micro morphological properties. The colony, mycelial, basidiospore morphology of fungal isolate was evidence of Basidiomycetes family. 18S rRNA gene of Ganoderma sp. DKR1 was sequenced by automated gene sequencer. Sequence were submitted NCBI and in accession number is GQ495094. The phylogenetic analysis of 18S rRNA gene of Ganoderma was compared with other species of Ganoderma and identified as Ganoderma sp. DKR1. The secondary structure and restriction sites present in the 18S RNA gene were predicted using bioinformatics tools. The medicinal mushroom was isolated from hard woods of C. equiestifolia and M. tinctoria. Further, Ganoderma sp. DKR1 was subjected to antibacterial activity which showed inhibition against Staphylococcus aureus and Micrococcus sp.
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